Creator/Principal investigator(s)
Rebecca Andersson
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Anna-Maria Eisele
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Sarah Hanzén
- Cochlear Nordic AB
Katarina Vielfort
- Umeå University, Institute of Molecular Biology
David Öling
- AstraZeneca, Mölnlycke, Sweden, Discovery Biology, Discovery Sciences, Innovative Medicines and Early Development Biotech Unit
Description
Language
English
Research principal
Responsible department/unit
Institute of Biomedicine, Department of Microbiology and Immunology
Data contains personal data
No
Unit of analysis
Population
Saccharomyces cerevisiae (Baker's yeast)
Study design
Experimental study
Preclinical study
Sampling procedure
Time period(s) investigated
2012 – 2020
Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, Johansson G, Gustafsson T, Kvint K, Nyström T. Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. bioRxiv. 2020 Jun 29. Available from: https://www.biorxiv.org/content/10.1101/2020.06.25.170670v2.full
DOI:
https://doi.org/10.1101/2020.06.25.170670
Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, et al. (2021) Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. PLoS Genet 17(1): e1008951.
DOI:
https://doi.org/10.1371/journal.pgen.1008951
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Description
Fluorescence microscopy of live yeast cells at mid-exponential growth. All strains produce the aggregate marker protein gus1-3-GFP or the metacaspase Mca1 tagged with GFP (Mca1-GFP)The dataset was collected through fluorescence microscopy.
The image files are provided in Carl Zeiss Image format (.czi).
Version 1
https://doi.org/10.5878/8wxm-8r38
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Data format / data structure
Still image
Creator/Principal investigator(s)
Sarah Hanzén
- Cochlear Nordic AB
Katarina Vielfort
- Umeå University, Institute of Molecular Biology
Rebecca Andersson
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Thomas Nyström
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
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Description
Fluorescence microscopy of live yeast cells at mid-exponential growth before stress, directly after 30 minutes of heat shock, and 20, 40 and 60 minutes after heat shock. The yeast is grown in complete synthetic medium with 2 % glucose as carbon source. All strains produce the aggregate marker protein guk1-7-GFP.The dataset was collected through fluorescence microscopy.
The image files are provided in Carl Zeiss Image format (.czi).
Version 1
https://doi.org/10.5878/8r0v-jk54
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Data format / data structure
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Creator/Principal investigator(s)
Rebecca Andersson
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Thomas Nyström
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
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Description
Fluorescence microscopy of live yeast cells at mid-exponential growth before and after 30 minutes of heat shock. All strains produce the Hsp70 chaperone protein Ssa4 tagged with GFP (Ssa4-GFP) and the metacaspase Mca1 tagged with RFP (Mca1-RFP).The dataset was collected through fluorescence microscopy.
The image files are provided in Carl Zeiss Image format (.czi).
Version 1
https://doi.org/10.5878/hvf7-3j18
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Data format / data structure
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Creator/Principal investigator(s)
Katarina Vielfort
- Umeå University, Institute of Molecular Biology
Rebecca Andersson
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Thomas Nyström
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
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Description
Fluorescence microscopy of live yeast cells at mid-exponential growth and after 30 minutes of heat shock. The cells were grown in complete synthetic media lacking histidine (CSM-HIS) with 2 % galactose as carbon source. All strains carry a plasmid expressing GFP-HSP104 under the control of the GAL-promoter.The dataset was collected through fluorescence microscopy.
The image files are provided in Carl Zeiss Image format (.czi).
Version 1
https://doi.org/10.5878/gv2g-xc30
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Data format / data structure
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Creator/Principal investigator(s)
Sarah Hanzén
- Cochlear Nordic AB
Rebecca Andersson
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Thomas Nyström
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
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Description
Timelapse microscopy of yeast cells during recovery after 30 minutes of heat shock. The yeast was cultivated in complete synthetic media with 2 % glucose as carbon source. All strains produce the misfolding protein guk1-7-GFP.The dataset was collected through timelapse microscopy of the same yeast cells for 2 hours.
The image files are provided in Carl Zeiss Image format (.czi).
Version 1
https://doi.org/10.5878/wj4m-y925
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Data format / data structure
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Creator/Principal investigator(s)
Sarah Hanzén
- Cochlear Nordic AB
Rebecca Andersson
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Thomas Nyström
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
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Description
Fluorescence microscopy of live yeast cells at mid-exponential growth and after 30 minutes of heat shock. The cells were grown in complete synthetic media lacking histidine and leucine with 2 % galactose as carbon source. All strains carry two plasmids; one expressing GFP-HSP104 under the control of the GAL-promoter and one expressing SSA1, SSA4 or SSA1/SSA4 chimaeras (alternatively an empty plasmid) under the control of the GPD-promoter.
The dataset was collected through fluorescence microscopy
Version 1
https://doi.org/10.5878/mez1-3z30
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Data format / data structure
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Creator/Principal investigator(s)
Rebecca Andersson
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Sarah Hanzén
- Cochlear Nordic AB
Katarina Vielfort
- Umeå University, Institute of Molecular Biology
Thomas Nyström
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Description
Fluorescence microscopy of live yeast cells at mid-exponential growth before stress, directly after 30 minutes of heat shock and 40 minutes after heat shock. The yeast is grown in complete synthetic medium lacking uracil with 2 % glucose as carbon source. All strains produce the aggregate marker protein guk1-7-GFP and carry a plasmid expressing SIK1-RFP under the control of the GPD-promoter.
The dataset was collected through fluorescence microscopy.
The image files are provided in Carl Zeiss Ima
Version 1
https://doi.org/10.5878/3cxh-6367
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Data format / data structure
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Creator/Principal investigator(s)
Rebecca Andersson
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Thomas Nyström
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Download data
Description
Fluorescence microscopy of live yeast cells at mid-exponential growth. The yeast is grown in complete synthetic medium with 2 % glucose as carbon source. All strains produce the aggregate marker protein guk1-7-GFP.The dataset was collected through fluorescence microscopy.
The image files are provided in Carl Zeiss Image format (.czi).
Version 1
https://doi.org/10.5878/d0ya-5n21
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Data format / data structure
Still image
Creator/Principal investigator(s)
Katarina Vielfort
- Umeå University, Institute of Molecular Biology
Rebecca Andersson
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Thomas Nyström
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
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Description
WT ssa12DD and ssa12DDSSA4OE: Western blots of SDS-PAGE gels with protein extracts from yeast in mid-exponential growth. The yeast was grown in complete synthetic media with 2 % glucose as carbon source.
WT ssa12DD w chimaera plasmids: Western blots of SDS-PAGE gels with protein extracts from yeast in mid-exponential growth before and after 30 minutes of heat shock. The yeast was grown in complete synthetic media lacking leucine with 2 % glucose as carbon source.
The images are scans of wester
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https://doi.org/10.5878/rq33-3f44
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Creator/Principal investigator(s)
Rebecca Andersson
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Thomas Nyström
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
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Description
Fluorescence microscopy of fixated yeast cells at mid-exponential growth. The samples have been stained with antibodies against Hsp42 (primary antibody: rabbit anti Hsp42, secondary antibody: goat anti rabbit Alexa Flour488) and counterstained with DAPI (datasets from 170505 and 170608) and antibodies against Actin (primary antibody: mouse anti- Act1, secondary antibody: goat anti mouse Alexa Flour 568) (dataset from 170518) or nucleoporin complexes (dataset from 170608) (primary antibody: mouse
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https://doi.org/10.5878/a3kq-6708
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Creator/Principal investigator(s)
Katarina Vielfort
- Umeå University, Institute of Molecular Biology
Rebecca Andersson
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Thomas Nyström
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
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Description
Images of two dimensional protein gels with protein extracts from wild type and Hsp70 mutant yeast strains in mid-exponential growth phase. The yeast were grown in rich medium (YP) with 2 % glucose as carbon source. The images have been aligned and the background has been removed.The dataset was collected through imaging of two dimensional protein gels.
The image files are provided in TIFF format.
Version 1
https://doi.org/10.5878/9xxe-g981
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Data format / data structure
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Creator/Principal investigator(s)
David Öling
- AstraZeneca, Mölnlycke, Sweden, Discovery Biology, Discovery Sciences, Innovative Medicines and Early Development Biotech Unit
Rebecca Andersson
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Thomas Nyström
- University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology