10.5878/d0ya-5n21
Vielfort, Katarina
Katarina
Vielfort
0000-0002-9988-6336
Institute of Molecular Biology, Umeå University
Andersson, Rebecca
Rebecca
Andersson
0000-0003-3765-5038
Institute of Biomedicine, Department of Microbiology and Immunology, University of Gothenburg
Nyström, Thomas
Thomas
Nyström
0000-0001-5489-2903
Institute of Biomedicine, Department of Microbiology and Immunology, University of Gothenburg
Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Hsp70-mutant yeast strains with the misfolding marker protein guk1-7-GFP and with or without an intact HSP104-allele, imaged during mid-exponential growth
Studier av det cytosoliska Hsp70-systemet från jäst och dess roll i proteinhomeostas och reglering av livslängd - Hsp70-mutanta jäststammar med det felveckande markörproteinet guk1-7-GFP samt med eller utan intakt HSP104-allel, avbildade under mitten av den exponentiella tillväxtfasen
University of Gothenburg
2020
Molecular Chaperones
Molekylära chaperoner
HSP70 Heat-Shock Proteins
HSP70-värmechockproteiner
Proteostasis Deficiencies
Proteostasbrist
Proteins
Proteiner
Heat-Shock Proteins
Värmechockproteiner
Metabolic Diseases
Ämnesomsättningssjukdomar
Nutritional and Metabolic Diseases
Näringsrubbningar och ämnesomsättningssjukdomar
Amino Acids, Peptides, and Proteins
Aminosyror, peptider och proteiner
Biochemistry and Molecular Biology
Biokemi och molekylärbiologi
Cell Biology
Cellbiologi
Microbiology
Mikrobiologi
Cell and Molecular Biology
Cell- och molekylärbiologi
Biological Sciences
Biologi
Basic Medicine
Medicinska och farmaceutiska grundvetenskaper
Natural Sciences
Naturvetenskap
Medical and Health Sciences
Medicin och hälsovetenskap
2020-12-16
2019-04-23/2019-04-25
2012/2020
eng
10.5878/dhrt-4t81
10.1101/2020.06.25.170670
10.1371/journal.pgen.1008951
1.58 GiB
1
Creative Commons Attribution 4.0 International (CC BY 4.0)
Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.
Fluorescence microscopy of live yeast cells at mid-exponential growth. The yeast is grown in complete synthetic medium with 2 % glucose as carbon source. All strains produce the aggregate marker protein guk1-7-GFP.
The dataset was collected through fluorescence microscopy.
The image files are provided in Carl Zeiss Image format (.czi).
Vi har systematiskt studerat vilka funktioner hos de cytosoliska molekylära chaperonerna av klassen Hsp70 hos jäst som krävs för att upprätthålla en normal livslängd.
Fluorescensmikroskopibilder av levande jästceller i mitten av den exponentiella tillväxtfasen. Jästen är odlad i komplett syntetiskt medium med 2 % glukos som kolkälla. Alla stammar producerar det felveckade markörproteinet guk1-7-GFP.
Datasetet har samlats in genom fluorescensmikroskopi.
Bildfilerna är i Carl Zeiss Image-format (.czi).
Knut and Alice Wallenberg Foundation
https://ror.org/004hzzk67