Feline microRNA transcriptome in whole blood in 6 healthy cats and in 6 cats with hypertrophic cardiomyopathy

SND-ID: 2021-334

Creator/Principal investigator(s)

Sofia Hanås - Swedish University of Agricultural Sciences, Department of Clinical Sciences orcid

Julie Lorent - Science for Life Laboratory

Åsa Ohlsson - Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics

Description

Aim to characterize differentially expressed miRNAS between healthy Norwegian Forest cats and healthy Domestic Shorthair cats, and to compare with cats with hypertropic cardiomyopathy (HCM)
Six neutered healthy cats, three male domestic cats (DOM) and one male and two female Norwegian Forest (NF) cats were included. Each healthy cat was matched (breed, sex, age, body-weight and body condition score) with a cat with HCM.
The dataset includes the miRDeep2 report, rawcounts miRNAs, differentially expressed miRNAs for contrasts compared using DESeq2, human and feline target genes producing messenger RNA (mRNA) and gene ontology analysis (GO) for these 12 cats.

Language

English

Research principal, contributors, and funding

Principal's reference number

SLU.kv.2021.4.4-197

Funding 1

  • Funding agency: Agria and the Swedish Kennel Club’s research fund
  • Funding agency's reference number: Projektnummer N2013-0025, P2014-0014, P2015-0004 och P2016-0003.
  • Project name on the application: Felina cirkulerande biomarkörer för hjärtsjukdom hos katt

Funding 2

  • Funding agency: SLU Companion Animals Research Fund
  • Project name on the application: Cirkulerande biomarkörer hos katter med och utan hjärtsjukdom

Funding 3

  • Funding agency: Sveland Research Fund
  • Project name on the application: Cirkulerande biomarkörer hos katter med hjärtsjukdom

Funding 4

  • Funding agency: Foundation Strömsholms Djursjukvård
  • Project name on the application: Cirkulerande biomarkörer hos katter med och utan hjärtsjukdom
Protection and ethical review

Data contains personal data

No

Ethics Review

Uppsala - Ref. C137/13

Method and time period

Unit of analysis

Population

12 cats, 2 breeds 6 cat of each breed (Norwegian Forest cats and Domestic cats), within each breed 3 healthy and three cats with hypertrophic cardiomyopathy (HCM)

Study design

Observational study

Description of study design

Six neutered healthy cats, three male domestic shorthair (DSH) and one male and two female NF cats were selected and matched with respect to breed, sex, age, and bodyweight with a cat with hypertrophic cardiomyopathy (HCM). One of the healthy DSH cats was matched with a Domestic Longhair cat (DLH), thus the DSH and DLH are hence called domestic (DOM) for the cats with preclinical HCM. Inclusion criteria were apparently healthy NF, and DSH cats, 1–14 years, with normal echocardiograms were included, as were cats of these two breed-groups with preclinical HCM. Diagnosis of HCM was based on characteristic findings on an echocardiogram. No cats receiving medical treatment were allowed into the study.

Sampling procedure

Other
Total RNA was extracted from WB, filtered for small RNA, and sequenced. Identification and prediction of miRNAs were performed with miRDeep2. Differential expression analysis of miRNAs was performed with DESeq2. The effect of breed was evaluated for healthy cats. The effect of preclinical HCM was evaluated for all cats and within breed. IntaRNA and miRDB was used for target-prediction, and DAVID for evaluation of gene ontology and gene enrichment.
Geographic coverage
Publications
Dataset
Feline microRNA transcriptome in whole blood in 6 healthy cats and in 6 cats with preclinical hypertrophic cardiomyopathy

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Associated documentation

Description

Whole blood was collected in PAXgene blood RNA System frozen and stored in -20 °C until date of RNA-extraction for a median storage time of 177 days. Total RNA was extracted. Samples with an RNA integrity number (RIN)-value of 7.7 or higher were included in the study. Libraries were prepared and quantified and normalized prior sequencing. Paired-end sequencing data was generated. Bioformatic data processing and count genertion of known and novel miRNAs in cats were identified using miRDeep2. Ma

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Version 1

Citation

Sofia Hanås, Julie Lorent, Åsa Ohlsson. Swedish University of Agricultural Sciences, Department of Clinical Sciences (2022). Feline microRNA transcriptome in whole blood in 6 healthy cats and in 6 cats with preclinical hypertrophic cardiomyopathy. Swedish National Data Service. Version 1. https://doi.org/10.5878/ex7j-1q61

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Data format / data structure

Numeric

Text

Other

Creator/Principal investigator(s)

Sofia Hanås - Swedish University of Agricultural Sciences, Department of Clinical Sciences orcid

Julie Lorent - Science for Life Laboratory

Åsa Ohlsson - Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics

Data collection

  • Mode of collection: Measurements and tests
  • Time period(s) for data collection: 2014-12-04–2016-06-13
  • Source of the data: Research data

Variables

6

Number of individuals/objects

12

Published: 2022-01-07