Creator/Principal investigator(s)
Sofia Hanås
- Swedish University of Agricultural Sciences, Department of Clinical Sciences
Julie Lorent - Science for Life Laboratory
Åsa Ohlsson - Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics
Description
Six neutered healthy cats, three male domestic cats (DOM) and one male and two female Norwegian Forest (NF) cats were included. Each healthy cat was matched (breed, sex, age, body-weight and body condition score) with a cat with HCM.
The dataset includes the miRDeep2 report, rawcounts miRNAs, differentially expressed miRNAs for contrasts compared using DESeq2, human and feline target genes producing messenger RNA (mRNA) and gene ontology analysis (GO) for these 12 cats.
Language
English
Research principal
Principal's reference number
SLU.kv.2021.4.4-197
Responsible department/unit
Department of Clinical Sciences
Data contains personal data
No
Ethics Review
Uppsala - Ref. C137/13
Unit of analysis
Population
12 cats, 2 breeds 6 cat of each breed (Norwegian Forest cats and Domestic cats), within each breed 3 healthy and three cats with hypertrophic cardiomyopathy (HCM)
Study design
Observational study
Description of study design
Six neutered healthy cats, three male domestic shorthair (DSH) and one male and two female NF cats were selected and matched with respect to breed, sex, age, and bodyweight with a cat with hypertrophic cardiomyopathy (HCM). One of the healthy DSH cats was matched with a Domestic Longhair cat (DLH), thus the DSH and DLH are hence called domestic (DOM) for the cats with preclinical HCM. Inclusion criteria were apparently healthy NF, and DSH cats, 1–14 years, with normal echocardiograms were included, as were cats of these two breed-groups with preclinical HCM. Diagnosis of HCM was based on characteristic findings on an echocardiogram. No cats receiving medical treatment were allowed into the study.
Sampling procedure
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Associated documentation
Description
Whole blood was collected in PAXgene blood RNA System frozen and stored in -20 °C until date of RNA-extraction for a median storage time of 177 days. Total RNA was extracted. Samples with an RNA integrity number (RIN)-value of 7.7 or higher were included in the study. Libraries were prepared and quantified and normalized prior sequencing. Paired-end sequencing data was generated. Bioformatic data processing and count genertion of known and novel miRNAs in cats were identified using miRDeep2. Ma
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https://doi.org/10.5878/ex7j-1q61
Citation
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Data format / data structure
Numeric
Text
Other
Creator/Principal investigator(s)
Sofia Hanås
- Swedish University of Agricultural Sciences, Department of Clinical Sciences
Julie Lorent - Science for Life Laboratory
Åsa Ohlsson - Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics
Variables
6
Number of individuals/objects
12