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Agilent gene expression microarray raw data for STO-3

SND-ID: 2021-340

Overview
Data and documentation

Creator/Principal investigator(s)

Annelie Johansson - Karolinska Institutet, Department of Oncology-Pathology

Linda Lindström - Karolinska Institutet, Department of Oncology-Pathology

Description

Agilent microarray profiling of primary breast tumors in the Stockholm tamoxifen trial (STO-3) was performed in 2014. The STO-3 trial enrolled 1780 postmenopausal lymph node-negative patients with tumors smaller than or equal to 30 mm and were randomized to tamoxifen vs no endocrine therapy. Molecular analysis was possible for 808 patients with available formalin-fixed paraffin-embedded (FFPE) tissue blocks from the primary breast cancer tumor. Eighty-one patients were excluded from analysis due to insufficient invasive tumor cells, leaving 727 samples available for further analysis. Gene expression data were independently generated using custom-designed arrays, Agilent Technologies (CA, USA), containing approximately 32.1K probes, representing approximately 21.5K unique genes from FFPE breast cancer tumor tissue. 652 of 727 breast cancer tumors passed the RNA quality check according to the diagnostic quality model and were used in the analysis, of which 538 were ER-positive.

Language

English

Research principal, contributors, and funding

Research principal

Karolinska Institutet

Responsible department/unit

Department of oncology-pathology

Protection and ethical review

Data contains personal data

Yes

Sensitive personal data

Yes

Type of personal data

Pseudonymised data

Code key exists

Yes

Ethics Review

Stockholm - Ref. 2017/2066-32, 97-451, 76-51.

The STO-3 trial was approved by the ethics committee at Karolinska Institutet and participants provided oral consent. The trial was conducted at the Regional Cancer Center Stockholm-Gotland, Sweden, and began in 1976

Method and time period

Population

Breast cancer patients diagnosed in Stockholm 1976-1990

Study design

Randomised controlled trial (RCT)

Biobank is connected to the study

This study has used existing samples from a scientific collection or biobank

Scientific collection or biobank name: Biobank Sverige, Stockholm tamoxifenstudierna (STO)

Type(s) of sample: formalin-fixed paraffin-embedded (FFPE) primary breast tumors

Geographic coverage

Geographic spread

Geographic location: Stockholm County

Topic and keywords

Research area

Genetics, Cancer and Oncology (The Swedish standard of fields of research 2011)

Keywords

breast neoplasms, tamoxifen, gene expression, clinical trial

Publications

Johansson & Lindström et al., Clinical and Molecular Characteristics of ER-Positive Ultralow Risk Breast Cancer Tumors Identified by the 70-Gene Signature, International Journal of Cancer, 2022
DOI: https://doi.org/10.1002/ijc.33969

If you have published anything based on these data, please notify us with a reference to your publication(s). If you are responsible for the catalogue entry, you can update the metadata/data description in DORIS.

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Dataset

Agilent gene expression microarray raw data for STO-3

Associated documentation

List_of_probes_STO3_Agilent_microarray_gMeanSignals_652.txt

Description

The datasetet consists of a table in one (1) txt file containing: Gene expression data (Agilent custom 32K microarray) generated in 2014 for 652 primary tumors from the STO-3 trial. The variables are the 31195 probes included in the Agilent microarray used to generate the data.
File name: STO3_Agilent_microarray_gMeanSignals_652.txt

The 31195 probes included in the Agilent microarray used to generate the data are listed and mapped to gene names in an annotation file.
Annotation file name: List_

Version 1

2022-03-02
https://doi.org/10.5878/3vxa-3c28

Citation

Annelie Johansson, Linda Lindström. Karolinska Institutet (2022). Agilent gene expression microarray raw data for STO-3. Swedish National Data Service. Version 1. https://doi.org/10.5878/3vxa-3c28

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Data format / data structure

Numeric

Creator/Principal investigator(s)

Annelie Johansson - Karolinska Institutet, Department of Oncology-Pathology

Linda Lindström - Karolinska Institutet, Department of Oncology-Pathology

Data collection

Mode of collection: Registry extract and/or access to biobank sample
Time period(s) for data collection: 2013–2014
Source of the data: Biological samples

Variables

31195

Number of individuals/objects

652

Published: 2022-03-02

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