Detection of multiple fish species in the diet of the invasive round goby reveals new trophic interactions in the Baltic Sea

SND-ID: 2023-73

Creator/Principal investigator(s)

Isa Wallin Kihlberg - Swedish University of Agricultural Sciences orcid

Description

The purpose of the study was to investigate round goby diet in two geographically distant areas in the Baltic Sea in two consecutive years, using two different methods. We collected round goby using fyke nets and gill nets in May and June 2018-2019 in Karlskrona in the southern Baltic Sea and on Åland in the northern Baltic Sea. The data set contains data of round goby diet, collected through both visual stomach content analysis (VSCA) and DNA metabarcoding. In VSCA, we dissected round goby and determined the prey items to the lowest possible taxonomic level, counted the individual prey/prey groups and estimated their relative proportions in each sample. In DNA metabarcoding, we used the 12S marker for detection of fish species in round goby diet, and the COI marker for an estimation of the proportions of fishes vs. invertebrates in the diet. DNA from round goby stomach samples was amplified using PCR and sequenced on the Illumina MiSeq platform. The output is then provided as number of sequences per prey species per sample, which roughly corresponds to relative biomass per prey species per sa

... Show more..

Language

English

Research principal, contributors, and funding

Principal's reference number

SLU.aqua.2023.4.4.IÄ-1

Responsible department/unit

Department of Aquatic Resources

Contributor(s)

Örjan Östman - Swedish University of Agricultural Sciences, Department of Aquatic Resources orcid

Ann-Britt Florin - Swedish University of Agricultural Sciences, Department of Aquatic Resources orcid

Karl Lundström - Swedish University of Agricultural Sciences, Department of Aquatic Resources orcid

Funding 1

  • Funding agency: Swedish Agency for Marine and Water Management rorId
  • Funding agency's reference number: Hav Dnr 3484-21
  • Project name on the application: DNA-metabarcoding av diet hos den invasiva svartmunnade smörbulten

Funding 2

  • Funding agency: Oscar and Lili Lamm memorial foundation rorId
  • Funding agency's reference number: FO 2017-0047
  • Project name on the application: Ekologiska effekter av den invasiva fisken svartmunnad smörbult i Östersjön

Funding 3

  • Funding agency: Swedish Board of Agriculture rorId
  • Funding agency's reference number: Jnr 2016-7109
  • Project name on the application: Svartmunnad smörbult i Blekinge och Kalmar län: utbredning, spridning, påverkan på ekosystem och potential som resurs
Protection and ethical review

Data contains personal data

No

Ethics Review

Uppsala - Ref. 5.8.18-07747/2018

Method and time period

Time period(s) investigated

2018-05-01 – 2018-06-30

2019-05-01 – 2019-06-30

Species and taxons

neogobius melanostomus

Geographic coverage

Geographic spread

Geographic location: Blekinge County, Åland Islands, Baltic Sea

Geographic description: In Blekinge the data was collected in three sampling locations outside of Karlskrona. In Åland the data was collected in Mariehamn (Västerhamn, by Elverket) and just outside Mariehamn (Lagneskär).

Topic and keywords
Publications
Dataset
Detection of multiple fish species in the diet of the invasive round goby reveals new trophic interactions in the Baltic Sea

Description

1. File Diet_data_RG_no_zoopl_200520_csv_1 (for RDA and relative prey proportions in VSCA). 346 rows, 50 columns.

2. File rg_dna_comparison_VSCA_COI_csv_1 (for visualization of fish and invertebrate proportions in DNA (COI) and visual stomach content analysis). 17 rows, 6 columns.

3. File RG_DNA_metabarcoding_12S_2018_2019_csv_1 (for RDA and relative prey proportions in DNA metabarcoding). 109 rows, 39 columns.

4. File RG_DNA_metabarcoding_FO_csv_1 (for visualization of frequency of occurr

... Show more..

Version 1

Citation

Isa Wallin Kihlberg. Swedish University of Agricultural Sciences (2023). Detection of multiple fish species in the diet of the invasive round goby reveals new trophic interactions in the Baltic Sea. Swedish National Data Service. Version 1. https://doi.org/10.5878/m5m1-br15

Download citation

Data format / data structure

Numeric

Creator/Principal investigator(s)

Isa Wallin Kihlberg - Swedish University of Agricultural Sciences orcid

Data collection

  • Mode of collection: Observation
  • Description of the mode of collection: Fishing with fyke nets and gill nets, visual stomach content analysis and DNA metabarcoding (12S and COI markers)
  • Time period(s) for data collection: 2018-05-01–2019-06-30
  • Data collector: Swedish University of Agricultural Sciences
  • Instrument:
  • Sample: Stomach content sample
    Remains of prey items found in round goby stomachs
  • Sample: DNA samples
    Stool collection tubes with buffer solution with remains of prey items from stomach content
  • Source of the data: Biological samples
  • Temporal resolution: 2 year
  • Spatial resolution: 515 kilometres

License

Creative Commons  Attribution 4.0 International (CC BY 4.0)
Published: 2023-04-28