Creator/Principal investigator(s)
Isa Wallin Kihlberg
- Swedish University of Agricultural Sciences
Description
The purpose of the study was to investigate round goby diet in two geographically distant areas in the Baltic Sea in two consecutive years, using two different methods. We collected round goby using fyke nets and gill nets in May and June 2018-2019 in Karlskrona in the southern Baltic Sea and on Åland in the northern Baltic Sea. The data set contains data of round goby diet, collected through both visual stomach content analysis (VSCA) and DNA metabarcoding. In VSCA, we dissected round goby and determined the prey items to the lowest possible taxonomic level, counted the individual prey/prey groups and estimated their relative proportions in each sample. In DNA metabarcoding, we used the 12S marker for detection of fish species in round goby diet, and the COI marker for an estimation of the proportions of fishes vs. invertebrates in the diet. DNA from round goby stomach samples was amplified using PCR and sequenced on the Illumina MiSeq platform. The output is then provided as number of sequences per prey species per sample, which roughly corresponds to relative biomass per prey species per sa
... Show more..Language
English
Research principal
Principal's reference number
SLU.aqua.2023.4.4.IÄ-1
Responsible department/unit
Department of Aquatic Resources
Data contains personal data
No
Ethics Review
Uppsala - Ref. 5.8.18-07747/2018
Time period(s) investigated
2018-05-01 – 2018-06-30
2019-05-01 – 2019-06-30
Species and taxons
neogobius melanostomus
Geographic spread
Geographic location: Blekinge County, Åland Islands, Baltic Sea
Geographic description: In Blekinge the data was collected in three sampling locations outside of Karlskrona. In Åland the data was collected in Mariehamn (Västerhamn, by Elverket) and just outside Mariehamn (Lagneskär).
Research area
Keywords
Download data
Associated documentation
code_fish_diet_sessionInfo.txt
Metadata_10_data_ttest_rg_median_catch_1819_rev.tsv
Metadata_9_data_ttest_BMI_AL_1819.tsv
Metadata_8_data_ttest_BMI_KK_1819.tsv
Metadata_7_data_ttest_fish_KK_1819.tsv
Metadata_6_VSCA_prey_abund_vs_rg_catch.tsv
Metadata_4_RG_DNA_metabarcoding_FO.tsv
Metadata_5_DNA_seq_vs_rg_catch.tsv
Metadata_3_RG_DNA_metabarcoding_12S_2018_2019.tsv
... Show more..Description
1. File Diet_data_RG_no_zoopl_200520_csv_1 (for RDA and relative prey proportions in VSCA). 346 rows, 50 columns.
2. File rg_dna_comparison_VSCA_COI_csv_1 (for visualization of fish and invertebrate proportions in DNA (COI) and visual stomach content analysis). 17 rows, 6 columns.
3. File RG_DNA_metabarcoding_12S_2018_2019_csv_1 (for RDA and relative prey proportions in DNA metabarcoding). 109 rows, 39 columns.
4. File RG_DNA_metabarcoding_FO_csv_1 (for visualization of frequency of occurr
Version 1
https://doi.org/10.5878/m5m1-br15
Citation
Download citation
Data format / data structure
Numeric
Creator/Principal investigator(s)
Isa Wallin Kihlberg
- Swedish University of Agricultural Sciences