RNA sequencing data from patients with heart disease and controls

RNA sequencing analysis of atrial cardiac tissue from patients undergoing coronary artery bypass grafting (CABG) or aortic valve replacement (AVR) was performed and compared with atrial cardiac tissue from organ donors and purchased human atrial cardiac RNA.

RNA sequencing analysis was performed at the Genomics Core Facility
at University of Gothenburg, Sweden. All samples (ten controls,
ten CABG patients and ten AVR patients) were quality checked by the
RNA integrity number (RIN) using Tapestation 2200 RNA screenTape
(Agilent Technologies, Santa Clara, CA) and RNA concentration was
measured by NanoDrop (Thermo Fisher, Waltham, MA). RIN values
ranged between 6.6 and 9.0 for all samples. TruSeq Total Stranded RNA
kit with RiboZero (Gold) Sample Preparation Guide (15,031,048 Rev. E)
was used for RNA sample preparation (Illumina, San Diego, CA).
A total of 10 μl (~1 μg) RNA from each sample was used for library
preparation. Directly after depletion, a cleanup step was performed
using 110 ul of the RNAClean XP beads (Beckman Coulter, USA) for each
sample. The fragmentation step was performed for 8 min. 12 PCR cycles
were run for all samples.
Libraries were quantified and normalized with Qubit DNA HS Assay
kit (Life Technologies, Carlsbad, CA) and fragment size determined by
Tapestation 2200 (Agilent Technologies, Santa Clara, CA). The libraries
were pooled together by using the Illumina protocol for pooling and
sequenced with NovaSeq 6000 S1 (Illumina, San Diego, CA) for the read
length of 2 × 100 bp. Show less..