Validation of an air-liquid interface toxicological set-up using Cu, Pd and Ag well-characterized nanostructured aggregates and spheres

Creator/Principal investigator(s)

Christian Svensson - Faculty of Engineering, Lund University, Department of Design Sciences

Description

Systems for studying the toxicity of metal aggregates on the airways are normally not suited for evaluating the effects of individual particle characteristics. This study validates a set-up for toxicological studies of metal aggregates using an air-liquid interface approach.

The set-up used a spark discharge generator capable of generating aerosol metal aggregate particles and sintered near spheres. The set-up also contained an exposure chamber, The Nano Aerosol Chamber for In Vitro Toxicity (NACIVT). The system facilitates on-line characterization capabilities of mass mobility, mass concentration and number size distribution to determine the exposure. By dilution, the desired exposure level was controlled.

Primary and cancerous airway cells were exposed to copper (Cu), palladium (Pd) and silver (Ag) aggregates. For Cu and Pd an exposure of sintered aerosol particles were also produced. The doses of the particles was expressed as particle numbers, masses and surface areas. For the Cu, Pd and Ag aerosol particles, a range of mass surface concentrations on the air-liquid interface of 0.4-1

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Principal organisation

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Responsible department/unit

Lund University

Creator/Principal investigator(s)

Christian Svensson - Faculty of Engineering, Lund University, Department of Design Sciences

Identifiers

SND-ID: SND 0993

Description

Systems for studying the toxicity of metal aggregates on the airways are normally not suited for evaluating the effects of individual particle characteristics. This study validates a set-up for toxicological studies of metal aggregates using an air-liquid interface approach.

The set-up used a spark discharge generator capable of generating aerosol metal aggregate particles and sintered near spheres. The set-up also contained an exposure chamber, The Nano Aerosol Chamber for In Vitro Toxicity (NACIVT). The system facilitates on-line characterization capabilities of mass mobility, mass concentration and number size distribution to determine the exposure. By dilution, the desired exposure level was controlled.

Primary and cancerous airway cells were exposed to copper (Cu), palladium (Pd) and silver (Ag) aggregates. For Cu and Pd an exposure of sintered aerosol particles were also produced. The doses of the particles was expressed as particle numbers, masses and surface areas. For the Cu, Pd and Ag aerosol particles, a range of mass surface concentrations on the air-liquid interface of 0.4-1

... Show more..

Language

English

Time period(s) investigated

2011-01-01 — 2013-01-01

Unit of analysis

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Associated documentation

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Version 1.0

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Validation of an air-liquid interface toxicological set-up using Cu, Pd and Ag well-characterized nanostructured aggregates and spheres

Suggested citation

Christian Svensson. Lund University (2016). <em>Validation of an air-liquid interface toxicological set-up using Cu, Pd and Ag well-characterized nanostructured aggregates and spheres</em>. Swedish National Data Service. Version 1.0. <a href="https://doi.org/10.5878/002761">https://doi.org/10.5878/002761</a>

Creator/Principal investigator(s)

Christian Svensson - Faculty of Engineering, Lund University, Department of Design Sciences

Description

Physical and biological data from air liquid interface cellular exposure studies.

Data format / data structure

Numeric

Data collection

Time period(s) for data collection: 2011-01-01 — 2013-01-01

Source of the data: Biological samples, Other

Variables

64

Published: 2016-03-01
Last updated: 2016-06-23