The effect of inactin on kidney mitochondrial function and production of reactive oxygen species

Creator/Principal investigator(s)

Fredrik Palm - Uppsala University, Department of Cell Biology

Tomas Schiffer - Uppsala University, Department of Cell Biology

Description

Inactin is an anesthetic agent commonly used in rat studies, but is also shown to exert physiological effects such as reducing renal blood flow, glomerular filtration rate and depressing tubular transport capacity. It is unknown whether inactin also effects isolated kidney mitochondria which may be important when studying related topics in anaesthetized animals. The aim of this study was to evaluate whether inactin exerts effects on mitochondrial function and production of reactive oxygen species. Kidney mitochondrial function and reactive oxygen production after acutely (5 min) or longer (1.5 hour) anesthetizing rats with inactin was determined by using high-resolution respirometry.

Subject area

Medical and Health Sciences (The Swedish standard of fields of research 2011)

Principal organisation

Uppsala University

Accessibility status

Access to data through SND
Data are freely accessible

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SND 1068 Data and documentation.zip (302.5 KB)

Responsible department/unit

Uppsala University

Creator/Principal investigator(s)

Fredrik Palm - Uppsala University, Department of Cell Biology

Tomas Schiffer - Uppsala University, Department of Cell Biology

Identifiers

SND-ID: SND 1068

Description

Inactin is an anesthetic agent commonly used in rat studies, but is also shown to exert physiological effects such as reducing renal blood flow, glomerular filtration rate and depressing tubular transport capacity. It is unknown whether inactin also effects isolated kidney mitochondria which may be important when studying related topics in anaesthetized animals. The aim of this study was to evaluate whether inactin exerts effects on mitochondrial function and production of reactive oxygen species. Kidney mitochondrial function and reactive oxygen production after acutely (5 min) or longer (1.5 hour) anesthetizing rats with inactin was determined by using high-resolution respirometry.

Subject area

Medical and Health Sciences (The Swedish standard of fields of research 2011)

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Version 1.0

2018-11-12
https://doi.org/10.5878/6m1n-7j65

Download data

SND 1068 Data and documentation.zip (302.5 KB)

The effect of inactin on kidney mitochondrial function and production of reactive oxygen species

Suggested citation

Fredrik Palm, Tomas Schiffer. Uppsala University (2018). <em>The effect of inactin on kidney mitochondrial function and production of reactive oxygen species</em>. Swedish National Data Service. Version 1.0. <a href="https://doi.org/10.5878/6m1n-7j65">https://doi.org/10.5878/6m1n-7j65</a>

Creator/Principal investigator(s)

Fredrik Palm - Uppsala University, Department of Cell Biology

Tomas Schiffer - Uppsala University, Department of Cell Biology

Data format / data structure

Numeric

Published: 2018-11-12
Last updated: 2019-02-08