The effect of inactin on kidney mitochondrial function and production of reactive oxygen species

Creator/Principal investigator(s):

Fredrik Palm - Uppsala University, Department of Cell Biology

Tomas Schiffer - Uppsala University, Department of Cell Biology

Description:

Inactin is an anesthetic agent commonly used in rat studies, but is also shown to exert physiological effects such as reducing renal blood flow, glomerular filtration rate and depressing tubular transport capacity. It is unknown whether inactin also effects isolated kidney mitochondria which may be important when studying related topics in anaesthetized animals. The aim of this study was to evaluate whether inactin exerts effects on mitochondrial function and production of reactive oxygen species. Kidney mitochondrial function and reactive oxygen production after acutely (5 min) or longer (1.5 hour) anesthetizing rats with inactin was determined by using high-resolution respirometry.

Subject area:

Medical and Health Sciences (The Swedish standard of fields of research 2011)

Principal organisation:

Uppsala University

Accessibility status:

Access to data through SND
Data are freely accessible

Download data:

SND 1068 Data and documentation.zip (302.5 KB)

Responsible department/unit:

Uppsala University

Creator/Principal investigator(s):

Fredrik Palm - Uppsala University, Department of Cell Biology

Tomas Schiffer - Uppsala University, Department of Cell Biology

Identifiers:

SND-ID: SND 1068

Description:

Inactin is an anesthetic agent commonly used in rat studies, but is also shown to exert physiological effects such as reducing renal blood flow, glomerular filtration rate and depressing tubular transport capacity. It is unknown whether inactin also effects isolated kidney mitochondria which may be important when studying related topics in anaesthetized animals. The aim of this study was to evaluate whether inactin exerts effects on mitochondrial function and production of reactive oxygen species. Kidney mitochondrial function and reactive oxygen production after acutely (5 min) or longer (1.5 hour) anesthetizing rats with inactin was determined by using high-resolution respirometry.

Subject area:

Medical and Health Sciences (The Swedish standard of fields of research 2011)

Download metadata:

Version 1.0:

2018-11-12 doi:10.5878/6m1n-7j65

Download data:

SND 1068 Data and documentation.zip (302.5 KB)

The effect of inactin on kidney mitochondrial function and production of reactive oxygen species

Suggested citation:

Fredrik Palm, Tomas Schiffer. Uppsala University (2018). The effect of inactin on kidney mitochondrial function and production of reactive oxygen species. Swedish National Data Service. Version 1.0. https://doi.org/10.5878/6m1n-7j65

Creator/Principal investigator(s):

Fredrik Palm - Uppsala University, Department of Cell Biology

Tomas Schiffer - Uppsala University, Department of Cell Biology

Data format / data structure:

Numeric

Published: 2018-11-12
Last updated: 2019-02-08