Repeated lumbar punctures within 3 days may affect CSF biomarker levels

Creator/Principal investigator(s)

Martin Olsson - University of Gothenburg orcid

Johan Ärlig - University of Gothenburg

Jan Hedner - University of Gothenburg, the Institute of Medicine

Kaj Blennow - University of Gothenburg, Institute of Neuroscience and Physiology

Henrik Zetterberg - University of Gothenburg, Institute of Neuroscience and Physiology

Description

As an extension of a study on the relationship between sleep deprivation and cerebrospinal fluid (CSF) biomarkers for Alzheimer’s disease, we performed two Lumbar punctures (LPs) within three days in 13 healthy volunteers. Our aim was to investigate CSF biomarker dynamics in relation to sleep deprivation. An unexpected sharp rise in biomarker concentrations in the second sample made us consider an artifact and we therefore repeated the experiment, but without sleep restriction, in four additional individuals. A similar rise in biomarker levels were evident, suggesting an inherent methodological problem with repeated LPs.

Creator/Principal investigator(s)

Martin Olsson - University of Gothenburg orcid

Johan Ärlig - University of Gothenburg

Jan Hedner - University of Gothenburg, the Institute of Medicine

Kaj Blennow - University of Gothenburg, Institute of Neuroscience and Physiology

Henrik Zetterberg - University of Gothenburg, Institute of Neuroscience and Physiology

Identifiers

SND-ID: SND 1132

Description

As an extension of a study on the relationship between sleep deprivation and cerebrospinal fluid (CSF) biomarkers for Alzheimer’s disease, we performed two Lumbar punctures (LPs) within three days in 13 healthy volunteers. Our aim was to investigate CSF biomarker dynamics in relation to sleep deprivation. An unexpected sharp rise in biomarker concentrations in the second sample made us consider an artifact and we therefore repeated the experiment, but without sleep restriction, in four additional individuals. A similar rise in biomarker levels were evident, suggesting an inherent methodological problem with repeated LPs.

Language

English

Time period(s) investigated

2015-02-01 — 2016-02-29

Unit of analysis

Population

Healthy volunteers

Study design

Cross-over

Description of study design

Randomized crossover

Sampling procedure

Other
13 healthy volunteers recruited by posters on university campus. 20-40 years of age with no sleep disturbances. Body-Mass-index (BMI) < 30 kg/m2. No continual use of medication or relevant chronic diseases. Self-reported normal bedtime < 00.00, regular morning awakening hours 06.00-09.00, habitual sleep duration of between 6.5-8.5 hours and absence of sleep disturbances (such as chronic insomnia/daytime sleepiness/narcolepsy). Epworth Sleepiness Scale (ESS) score < 11.

Ethics Review

Gothenburg — Ref. 823-14

Biobank is connected to the study

Yes

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Publications

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Olsson M, Arlig J, Hedner J, Blennow K, Zetterberg H. Sleep deprivation and cerebrospinal fluid biomarkers for Alzheimer's disease. Sleep. 2018;41(5). https://doi.org/10.1093/sleep/zsy025
Link to article
DOI: https://doi.org/10.1093/sleep/zsy025

Olsson, M., Ärlig, J., Hedner, J. et al. Repeated lumbar punctures within 3 days may affect CSF biomarker levels. Fluids Barriers CNS 16, 37 (2019) doi:10.1186/s12987-019-0157-2
Link to article
DOI: https://doi.org/10.1186/s12987-019-0157-2

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Dataset 1

Repeated lumbar punctures within 3 days without sleep intervention

Citation

Martin Olsson, Johan Ärlig, Jan Hedner, Kaj Blennow, Henrik Zetterberg. University of Gothenburg, Institute of Neuroscience and Physiology (2019). <em>Repeated lumbar punctures within 3 days without sleep intervention</em>. Swedish National Data Service. Version 1.0. <a href="https://doi.org/10.5878/tpbk-k885">https://doi.org/10.5878/tpbk-k885</a>

Creator/Principal investigator(s)

Martin Olsson - University of Gothenburg orcid

Johan Ärlig - University of Gothenburg

Jan Hedner - University of Gothenburg, the Institute of Medicine

Kaj Blennow - University of Gothenburg, Institute of Neuroscience and Physiology

Henrik Zetterberg - University of Gothenburg, Institute of Neuroscience and Physiology

Description

Participants with controled normal sleep. Biomarker concentrations from each individual lumbar puncture.

Data format / data structure

Numeric

Variables

8

Number of individuals/objects

4

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Dataset 2

Repeated lumbar punctures. After sleep deprivation and 3 days later, after recovery sleep

Citation

Martin Olsson, Johan Ärlig, Jan Hedner, Kaj Blennow, Henrik Zetterberg. University of Gothenburg, Institute of Neuroscience and Physiology (2019). <em>Repeated lumbar punctures. After sleep deprivation and 3 days later, after recovery sleep</em>. Swedish National Data Service. Version 1.0. <a href="https://doi.org/10.5878/cavp-eh78">https://doi.org/10.5878/cavp-eh78</a>

Creator/Principal investigator(s)

Martin Olsson - University of Gothenburg orcid

Johan Ärlig - University of Gothenburg

Jan Hedner - University of Gothenburg, the Institute of Medicine

Kaj Blennow - University of Gothenburg, Institute of Neuroscience and Physiology

Henrik Zetterberg - University of Gothenburg, Institute of Neuroscience and Physiology

Description

Biomarker concentrations from each individual lumbar puncture. First puncture immediately after sleep deprivation. Second puncture three days later, after recovery sleep.

Data format / data structure

Numeric

Variables

8

Number of individuals/objects

13

Published: 2019-12-16