Repeated lumbar punctures within 3 days may affect CSF biomarker levels

Creator/Principal investigator(s):

Martin Olsson - University of Gothenburg orcid

Johan Ärlig - University of Gothenburg

Jan Hedner - University of Gothenburg, the Institute of Medicine

Kaj Blennow - University of Gothenburg, Institute of Neuroscience and Physiology

Henrik Zetterberg - University of Gothenburg, Institute of Neuroscience and Physiology

Description:

As an extension of a study on the relationship between sleep deprivation and cerebrospinal fluid (CSF) biomarkers for Alzheimer’s disease, we performed two Lumbar punctures (LPs) within three days in 13 healthy volunteers. Our aim was to investigate CSF biomarker dynamics in relation to sleep deprivation. An unexpected sharp rise in biomarker concentrations in the second sample made us consider an artifact and we therefore repeated the experiment, but without sleep restriction, in four additional individuals. A similar rise in biomarker levels were evident, suggesting an inherent methodological problem with repeated LPs.

Subject area:

Medical and Health Sciences (The Swedish standard of fields of research 2011)

Keywords:

Cerebrospinal Fluid, Cerebrospinal Fluid Proteins, Amyloid beta-Peptides, lumbar puncture, tau, cerebrospinal fluid proteins, CSF

Principal organisation:

University of Gothenburg

Accessibility status:

Access to data through SND
Data are freely accessible

Download data:

SND1132-001-V1.0.zip (8.69 KB)
Repeated lumbar punctures within 3 days without sleep intervention
SND1132-002-V1.0.zip (11.43 KB)
Repeated lumbar punctures. After sleep deprivation and 3 days later, after recovery sleep

Responsible department/unit:

Creator/Principal investigator(s):

Martin Olsson - University of Gothenburg orcid

Johan Ärlig - University of Gothenburg

Jan Hedner - University of Gothenburg, the Institute of Medicine

Kaj Blennow - University of Gothenburg, Institute of Neuroscience and Physiology

Henrik Zetterberg - University of Gothenburg, Institute of Neuroscience and Physiology

Identifiers:

SND-ID: SND 1132

Description:

As an extension of a study on the relationship between sleep deprivation and cerebrospinal fluid (CSF) biomarkers for Alzheimer’s disease, we performed two Lumbar punctures (LPs) within three days in 13 healthy volunteers. Our aim was to investigate CSF biomarker dynamics in relation to sleep deprivation. An unexpected sharp rise in biomarker concentrations in the second sample made us consider an artifact and we therefore repeated the experiment, but without sleep restriction, in four additional individuals. A similar rise in biomarker levels were evident, suggesting an inherent methodological problem with repeated LPs.

Language:

English

Time period(s) investigated:

2015-02-01 — 2016-02-29

Unit of analysis:

Population:

Healthy volunteers

Study design:

Cross-over

Description of study design:

Randomized crossover

Sampling procedure:

Other
13 healthy volunteers recruited by posters on university campus. 20-40 years of age with no sleep disturbances. Body-Mass-index (BMI) < 30 kg/m2. No continual use of medication or relevant chronic diseases. Self-reported normal bedtime < 00.00, regular morning awakening hours 06.00-09.00, habitual sleep duration of between 6.5-8.5 hours and absence of sleep disturbances (such as chronic insomnia/daytime sleepiness/narcolepsy). Epworth Sleepiness Scale (ESS) score < 11.

Ethics Review:

Gothenburg — Ref. 823-14

Biobank is connected to the study:

Yes

Subject area:

Medical and Health Sciences (The Swedish standard of fields of research 2011)

Keywords:

Cerebrospinal Fluid, Cerebrospinal Fluid Proteins, Amyloid beta-Peptides, lumbar puncture, tau, cerebrospinal fluid proteins, CSF

Download metadata:

Publications

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Olsson M, Arlig J, Hedner J, Blennow K, Zetterberg H. Sleep deprivation and cerebrospinal fluid biomarkers for Alzheimer's disease. Sleep. 2018;41(5). https://doi.org/10.1093/sleep/zsy025
Link to article

Olsson, M., Ärlig, J., Hedner, J. et al. Repeated lumbar punctures within 3 days may affect CSF biomarker levels. Fluids Barriers CNS 16, 37 (2019) doi:10.1186/s12987-019-0157-2
Link to article

If you have published anything based on these data, please notify us with a reference to your publication(s).

Version 1.0:

2019-11-29 doi:10.5878/tpbk-k885

Download data:

SND1132-001-V1.0.zip (8.69 KB)
Repeated lumbar punctures within 3 days without sleep intervention

Dataset SND 1132-001

Repeated lumbar punctures within 3 days without sleep intervention

Citation:

Martin Olsson, Johan Ärlig, Jan Hedner, Kaj Blennow, Henrik Zetterberg. University of Gothenburg, Institute of Neuroscience and Physiology (2019). Repeated lumbar punctures within 3 days without sleep intervention. Swedish National Data Service. Version 1.0. https://doi.org/10.5878/tpbk-k885

Description:

Participants with controled normal sleep. Biomarker concentrations from each individual lumbar puncture.

Data format / data structure:

Numeric

Variables:

8

Number of individuals/objects:

4

Version 1.0:

2019-11-29 doi:10.5878/cavp-eh78

Download data:

SND1132-002-V1.0.zip (11.43 KB)
Repeated lumbar punctures. After sleep deprivation and 3 days later, after recovery sleep

Dataset SND 1132-002

Repeated lumbar punctures. After sleep deprivation and 3 days later, after recovery sleep

Citation:

Martin Olsson, Johan Ärlig, Jan Hedner, Kaj Blennow, Henrik Zetterberg. University of Gothenburg, Institute of Neuroscience and Physiology (2019). Repeated lumbar punctures. After sleep deprivation and 3 days later, after recovery sleep. Swedish National Data Service. Version 1.0. https://doi.org/10.5878/cavp-eh78

Description:

Biomarker concentrations from each individual lumbar puncture. First puncture immediately after sleep deprivation. Second puncture three days later, after recovery sleep.

Data format / data structure:

Numeric

Variables:

8

Number of individuals/objects:

13