Cell to cell signaling via exosomes (transport of genetic material between cells via exosomes)

Creator/Principal investigator(s):

Hadi Valadi - University of Gothenburg, Avdelningen för reumatologi och inflammationsforskning orcid

Description:

In the human body, communication between cells takes place in several different ways. Exosomes are a type of extracellular vesicles (30-200 nm in diameter) that also participate in this process. Exosomes can be released from most cell types in the body and exist naturally in different body fluids, such as blood.

In 2007, we showed for the first time that exosomes also transport RNA molecules (both coding and non-coding RNA molecules) between cells (Valadi H. et al. Nature Cell Biology. 2007). The findings describe a new type of 'cell-cell' communication by which different cells send genetic messages to each other by secreting vesicles (exosomes) containing specific RNA molecules.

Our current studies aim to (a) identify cellular actors involved in translocation (packaging) of RNA into exosomes during their biosynthesis, (b) identify the mechanism by which exosomes are taken up by recipient cells/ tissues, and (c) ) discover a method to introduce exogenous/ therapeutic RNA into exosomes, to be used for in vivo transport of therapeutic RNA to different organs.

Principal organisation:

Download data:

SND1142-001-V1.0.zip (442.14 KB)
Identification of RNA-binding proteins in exosomes capable of interacting with different types of RNA: RBP-facilitated transport of RNAs into exosomes

Creator/Principal investigator(s):

Hadi Valadi - University of Gothenburg, Avdelningen för reumatologi och inflammationsforskning orcid

Identifiers:

SND-ID: SND 1142

ProjektID: Exosome RNA

Description:

In the human body, communication between cells takes place in several different ways. Exosomes are a type of extracellular vesicles (30-200 nm in diameter) that also participate in this process. Exosomes can be released from most cell types in the body and exist naturally in different body fluids, such as blood.

In 2007, we showed for the first time that exosomes also transport RNA molecules (both coding and non-coding RNA molecules) between cells (Valadi H. et al. Nature Cell Biology. 2007). The findings describe a new type of 'cell-cell' communication by which different cells send genetic messages to each other by secreting vesicles (exosomes) containing specific RNA molecules.

Our current studies aim to (a) identify cellular actors involved in translocation (packaging) of RNA into exosomes during their biosynthesis, (b) identify the mechanism by which exosomes are taken up by recipient cells/ tissues, and (c) ) discover a method to introduce exogenous/ therapeutic RNA into exosomes, to be used for in vivo transport of therapeutic RNA to different organs.

Language:

English

Time period(s) investigated:

2008-01-01 — ongoing

Geographic spread:

Geographic location: Sweden

Unit of analysis:

Population:

Currently, not available

Study design:

Experimental study

Preclinical study

Publications

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Maugeri M, Nawaz M, Papadimitriou A, Angerfors A, Camponeschi A, Na M, Hölttä M, Skantze P, Johansson S, Sundqvist M, Lindquist J, Kjellman T, Mårtensson IL, Jin T, Sunnerhagen P, Östman S, Lindfors L, Valadi H., Nature Communication. 2019 Sep 24;10(1):4333. https://doi.org/10.1038/s41467-019-12275-6
Link to article

Statello L, Maugeri M, Garre E, Nawaz M, Wahlgren J, Papadimitriou A, et al. (2018) Identification of RNA-binding proteins in exosomes capable of interacting with different types of RNA: RBP-facilitated transport of RNAs into exosomes. PLoS ONE 13(4): e0195969. https://doi.org/10.1371/journal.pone.0195969
Link to article

If you have published anything based on these data, please notify us with a reference to your publication(s).

Version 1.0:

Download data:

SND1142-001-V1.0.zip (442.14 KB)
Identification of RNA-binding proteins in exosomes capable of interacting with different types of RNA: RBP-facilitated transport of RNAs into exosomes

Identification of RNA-binding proteins in exosomes capable of interacting with different types of RNA: RBP-facilitated transport of RNAs into exosomes

Citation:

Hadi Valadi. University of Gothenburg, the Institute of Medicine (2020). Identification of RNA-binding proteins in exosomes capable of interacting with different types of RNA: RBP-facilitated transport of RNAs into exosomes. Swedish National Data Service. Version 1.0. https://doi.org/10.5878/6b1e-fs87

URL:

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0195969#sec025

Description:

RNA-binding proteins (RBPs) present in exosomes derived from HTB177 cells (NCI-H460 [H460] (ATCC: HTB-177) were identified as described below.

For more detailed description please see Statello L, Maugeri M, Garre E, Nawaz M, Wahlgren J, Papadimitriou A, et al. (2018) (doi: 10.1371/journal.pone.0195969. eCollection 2018). List of the identified proteins can be found in the section 'Data collection' as well as on the journal's website (https://journals.plos.org/plosone/article?id=10.1371/journal

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Data format / data structure:

Text

Data collection:

Mode of collection: Measurements and tests

Time period(s) for data collection: 2008

Source of the data: Research data