Modeling the Transport of Nuclear Proteins Along Single Skeletal Muscle Cells

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Creator/Principal investigator(s):

Ana Teixeira - Karolinska Institutet, Department of Medical Biochemistry and Biophysics orcid

Description:

The purpose of this study was to define the factors that control the distribution of nuclear proteins in multinucleated skeletal muscle cells (myotubes). Nuclear-targeted red fluorescent proteins of different molecular weights were used as a model protein. Changes in their distributions were quantified as a result of perturbations causing myotube hypertrophy, atrophy, and diminished nuclear import rates. A computational model was developed to simulate nuclear protein distribution using a combination of empirical values and data from the literature. Finally, the results from the model system were then validated using muscle-related transcription factors ARNT and Six1.

Responsible department/unit:

Karolinska Institutet, Department of Medical Biochemistry and Biophysics

Creator/Principal investigator(s):

Ana Teixeira - Karolinska Institutet, Department of Medical Biochemistry and Biophysics orcid

Contributor(s):

Christopher Grigsby - Karolinska Institutet, Department of Medical Biochemistry and Biophysics orcid

Identifiers:

SND-ID: SND 1146

Description:

The purpose of this study was to define the factors that control the distribution of nuclear proteins in multinucleated skeletal muscle cells (myotubes). Nuclear-targeted red fluorescent proteins of different molecular weights were used as a model protein. Changes in their distributions were quantified as a result of perturbations causing myotube hypertrophy, atrophy, and diminished nuclear import rates. A computational model was developed to simulate nuclear protein distribution using a combination of empirical values and data from the literature. Finally, the results from the model system were then validated using muscle-related transcription factors ARNT and Six1.

Language:

English

Unit of analysis:

Population:

Myotubes derived from primary mouse myoblasts

Study design:

Experimental study

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Modeling the transport of nuclear proteins along single skeletal muscle cells. Hermes Taylor-Weiner, Christopher L. Grigsby, Duarte M. S. Ferreira, José M. Dias, Molly M. Stevens, Jorge L. Ruas, Ana I. Teixeira. Proceedings of the National Academy of Sciences, Jan 2020, 201919600; DOI: 10.1073/pnas.1919600117
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DOI: 10.1073/pnas.1919600117

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Version 1.0:

Modeling the Transport of Nuclear Proteins Along Single Skeletal Muscle Cells

Suggested citation:

Ana Teixeira. Karolinska Institutet, Department of Medical Biochemistry and Biophysics (2020). Modeling the Transport of Nuclear Proteins Along Single Skeletal Muscle Cells. Swedish National Data Service. Version 1.0. https://doi.org/10.5878/2hjy-e586

Creator/Principal investigator(s):

Ana Teixeira - Karolinska Institutet, Department of Medical Biochemistry and Biophysics orcid

Description:

These data were generated to study the distribution of nuclear proteins within multi-nucleated skeletal muscle cells. Myotubes were produced by differentiating primary mouse myoblasts that were sparsely transfected to express a nuclear protein from a single nucleus. From the microscopy images, we measured the protein intensity in all nuclei along with the distance of each nucleus from the brightest one, which was assumed to be the transfected nucleus. We used these distribution profiles to study

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Published: 2020-02-19