Karolinska Institutet, Department of Medical Biochemistry and Biophysics
SND-ID: SND 1146
Unit of analysis
Myotubes derived from primary mouse myoblasts
Modeling the transport of nuclear proteins along single skeletal muscle cells. Hermes Taylor-Weiner, Christopher L. Grigsby, Duarte M. S. Ferreira, José M. Dias, Molly M. Stevens, Jorge L. Ruas, Ana I. Teixeira. Proceedings of the National Academy of Sciences, Jan 2020, 201919600; DOI: 10.1073/pnas.1919600117
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These data were generated to study the distribution of nuclear proteins within multi-nucleated skeletal muscle cells. Myotubes were produced by differentiating primary mouse myoblasts that were sparsely transfected to express a nuclear protein from a single nucleus. From the microscopy images, we measured the protein intensity in all nuclei along with the distance of each nucleus from the brightest one, which was assumed to be the transfected nucleus. We used these distribution profiles to study... Show more..
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