Variable,Explanation Site_ID,Unique ID for the 52 surveyed created wetlands (IDs are only related to this and referenced study). Abramis brama,Presence/absence of that fish species eDNA in a wetland Alburnus alburnus,Presence/absence of that fish species eDNA in a wetland Anguilla anguilla,Presence/absence of that fish species eDNA in a wetland Blicca bjoerkna,Presence/absence of that fish species eDNA in a wetland Carassius carassius,Presence/absence of that fish species eDNA in a wetland Cottus gobio,Presence/absence of that fish species eDNA in a wetland Cyprinus carpio,Presence/absence of that fish species eDNA in a wetland Esox lucius,Presence/absence of that fish species eDNA in a wetland Gasterosteus aculeatus,Presence/absence of that fish species eDNA in a wetland Gymnocephalus cernua,Presence/absence of that fish species eDNA in a wetland Lota lota,Presence/absence of that fish species eDNA in a wetland Oncorhynchus mykiss,Presence/absence of that fish species eDNA in a wetland Perca fluviatilis,Presence/absence of that fish species eDNA in a wetland Pungitius pungitius,Presence/absence of that fish species eDNA in a wetland Rutilus rutilus,Presence/absence of that fish species eDNA in a wetland Salmo trutta,Presence/absence of that fish species eDNA in a wetland Tinca tinca,Presence/absence of that fish species eDNA in a wetland Scardinius erythrophthalmus,Presence/absence of that fish species eDNA in a wetland Bufo bufo,Presence/absence of that amphibian species eDNA in a wetland Rana arvalis,Presence/absence of that amphibian species eDNA in a wetland Rana temporaria,Presence/absence of that amphibian species eDNA in a wetland Lissotriton vulgaris,Presence/absence of that amphibian species eDNA in a wetland Triturus cristatus,Presence/absence of that amphibian species eDNA in a wetland Collection date (YYYY-MM-DD),The day eDNA was collected from the wetland mL filtered,The millilitres of water sampled from the wetland was filtered to obtain the eDNA water temperature,The temperature of the water at the time of sampling (C). NA indicates missing data. Wetland size,"Water surface, including emergent water vegetation (ha)" Flooded area,"Adjacent grassland that can be flooded (%), defined by soil humidity and wet grassland vegetation (50 m buffer surrounding the water surface shore)" Year of creation (YYYY),"Year wetland was created. When this was not known, the year interval (max five years) was assigned based on aerial photos, and the midyear was used as an entry" Connectivity,Whether the wetland has ditches/streams that connect it to other water bodies (except for dams which are assumed to be isolated in terms of natural fish colonisation probability) Water vegetation,"Cover of wetland area by emergent water vegetation (e.g. Typha, Phragmites, Iris, etc.) and floating water vegetation (e.g. lilies, duckweed, algae, etc.) (%)" Landscape forest,Coverage of forest within 1 km buffer starting from the wetland shore (%)